Confirmation of Candidature - Develop an Integrated and Novel bio-Medium for the Production of Enzymes and Biofuel From Sorghum Grain Using Fungal Fermentation

This study focuses on developing a sustainable approach for low-cost biofuel production from sorghum grain, aiming to address climate change and reduce dependence on fossil fuels. Through fungal fermentation employing Aspergillus awamori and Aspergillus oryzae, hydrolysis of sorghum grain and bran will be carried out without relying on external carbon and nitrogen sources, facilitating a recycling process. Our research aimed to generate novel fermentation media rich in total reducing sugars (TRS), free amino nitrogen (FAN), total nitrogen, and a potent enzyme cocktail comprising α-amylase, glucoamylase, and protease. Investigation into particle size distribution (PSD) and moisture content effects on fungal fermentation efficiency in both solid-state fermentation (SSF) and submerged fermentation (SmF) will be conducted. pH dynamics during fermentation fluctuated from 6 to 3, with optimal conditions identified for enzyme production. Results revealed that larger particle sizes of sorghum grain yielded maximal TRS production (8.69 g/L) within 48 hours, while FAN production peaked at 96 hours (217.26 mg/L). Particle size (150-300µm) of sorghum flour exhibited maximum α-amylase production (2851.86 U/mL) at 168 hours among five different particle sizes of sorghum flour. These findings underscore the potential of sorghum-based biofuel production and the importance of optimizing fermentation processes for enhanced enzyme yields.

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